Exosomes are an emerging analyte class of microparticles rich in information related to the normal and pathological status of all cells in the human body.
Exosomes are nanovesicles around 30nm to 150nm in diameter and are secreted by most cells. These vesicles play an important role in cell communication and are carriers of a wide variety of proteins, mRNA, DNA and other signaling molecules. When exosomes released from one cell interact with neighboring cells, they exert certain physiological changes in the recipient cell. These changes are significant in diseased states such as cancer, where the exosomes secreted by cancer cells or tumors may be responsible for the immune suppression via inactivation of myeloid cells and the progression of cancer1,2
One of the main challenges in exosome studies is the characterization of exosomes to determine the efficiency of the isolation process. An ideal exosome isolate will have a hydrodynamic diameter of 30nm to 150nm and a high yield (particles/ml). Nanoparticle Tracking Analysis – NTA is a powerful method that can be used to characterize the exosome isolate based on the Brownian motion of the particles. The system analyzes the particles present in the sample and determines both the size in hydrodynamic diameter (nm) and the concentration of the sample (particles/ml).
NTA can be easily used to characterize your exosome sample with minimum sample volume of 1µl. Therefore, this is an ideal method to determine both the size and count of the exosome isolate, giving an estimate of the success of the isolation method with respect to exosome yield and size